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Promega luciferase reporter construct p2
Luciferase Reporter Construct P2, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+construct+p2/luciferase+reporter+construct+p2/ppr0199292-82-20-33
Average 90 stars, based on 1 article reviews
luciferase reporter construct p2 - by Bioz Stars, 2026-10
90/100 stars

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Transfection:

Article Title: Phosphorylation of Pit-1 by Cyclin-Dependent Kinase 5 at Serine 126 is Associated with Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporter construct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Article Title: Phosphorylation of Pit-1 by Cyclin-dependent Kinase 5 at Serine 126 is Associated With Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporterconstruct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s Page 6/16 instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Construct:

Article Title: Phosphorylation of Pit-1 by Cyclin-Dependent Kinase 5 at Serine 126 is Associated with Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporter construct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Article Title: Phosphorylation of Pit-1 by Cyclin-dependent Kinase 5 at Serine 126 is Associated With Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporterconstruct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s Page 6/16 instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Luciferase:

Article Title: Phosphorylation of Pit-1 by Cyclin-Dependent Kinase 5 at Serine 126 is Associated with Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporter construct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Article Title: Phosphorylation of Pit-1 by Cyclin-dependent Kinase 5 at Serine 126 is Associated With Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporterconstruct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s Page 6/16 instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Control:

Article Title: Phosphorylation of Pit-1 by Cyclin-Dependent Kinase 5 at Serine 126 is Associated with Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporter construct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Article Title: Phosphorylation of Pit-1 by Cyclin-dependent Kinase 5 at Serine 126 is Associated With Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporterconstruct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s Page 6/16 instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Plasmid Preparation:

Article Title: Phosphorylation of Pit-1 by Cyclin-Dependent Kinase 5 at Serine 126 is Associated with Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporter construct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).

Article Title: Phosphorylation of Pit-1 by Cyclin-dependent Kinase 5 at Serine 126 is Associated With Cell Proliferation and Poor Prognosis in Prolactinomas
Article Snippet: .. The cells were transfected with 0.2 μg of dual-luciferase reporterconstruct p1, or they were cotransfected with 0.2 μg of the luciferase reporter construct p2 and the internal control vector pRL-TK, pRL-SV40, or pRL-CMV (Promega, Madison, WI) at a ratio of 20:1 (reporter construct: control vector); transfections were performed using LipofectamineTM 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s Page 6/16 instructions. .. Five hours posttransfection, the transfection medium was removed and replaced with medium containing 6 μM curcumin (Sigma-Aldrich, St. Louis, MO) solubilized in 100% dimethyl sulfoxide (Sigma).



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Promega luciferase reporter construct p2
Luciferase Reporter Construct P2, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+construct+p2/luciferase+reporter+construct+p2/ppr0199292-82-20-33
Average 90 stars, based on 1 article reviews
luciferase reporter construct p2 - by Bioz Stars, 2026-10
90/100 stars
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